Many studies have being performed about in vitro function of CPDA-1 blood components, since CPDA-1 has replaced ACD-B in May, 1991 in Korea. Korean Red Cross blood center supplies platelet concentrates contained in bags made of PVC, which can be
stored
for maximum 3 days, but practically most blood banks use these platelet concentrates within 24 hours.
In order to investigate changes in morphology and function of CPDa-1 platelet concentrates during storage, the author performed in vitro platelet counts, MPV, PDW, bicarbonate concentration, pltelet aggregation response to ristocetin and
flowcytometric
patterns of platelet glycoprotein Ib on day 1, 2, 3 and 5 while mixing 70 cycle/min platelet agitator at 22℃. The results are as follows; First, platelet counts, MPV and PDW at day 3 were well maintained as 230×10E9/L, 7.7 and 10.8fL,
respectively. At
day 5, platelet counts were decreased to 877×10E9/L, MPV and PDW were increased to 9.8, 11.8fL, respectively. Second, pH and bicarbonate concentration were decreased during storage. And then, at day 5, they were 6.6, 3.4mmol/L, respectively.
Third,
platelet aggregation response to ristocetin and flcwoytometric patterns of glycoprotein Ib were well maintained at day 3, but at day 5, they were decreased correspondingly.
Consequently, we concluded that CPDA-1 platelet concentrated contained in PVC bags can be used for a maximum of 3 days without changes in morphology and function of platelets.
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